Goat Anti-Human IgG Fc (DyLight® 650) preadsorbed (ab98622)
Key features and details
- Goat Anti-Human IgG Fc (DyLight® 650) preadsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat Anti-Human IgG Fc (DyLight® 650) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
|
ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab98622 has been referenced in 8 publications.
- Kugathasan R et al. Deep mutagenesis scanning using whole trimeric SARS-CoV-2 spike highlights the importance of NTD-RBD interactions in determining spike phenotype. PLoS Pathog 19:e1011545 (2023). PubMed: 37535672
- Wang Z et al. 3D-organoid culture supports differentiation of human CAR+ iPSCs into highly functional CAR T cells. Cell Stem Cell 29:515-527.e8 (2022). PubMed: 35278370
- Guo H et al. ACE2-Independent Bat Sarbecovirus Entry and Replication in Human and Bat Cells. mBio 13:e0256622 (2022). PubMed: 36409074
- Wang Y et al. High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics. Sci Adv 7:N/A (2021). PubMed: 34117053
- Billerhart M et al. CD47-targeted cancer immunogene therapy: Secreted SIRPα-Fc fusion protein eradicates tumors by macrophage and NK cell activation. Mol Ther Oncolytics 23:192-204 (2021). PubMed: 34729396
- Kelly JH A single injection of CM1021, a long half-life hepatocyte growth factor mimetic, increases liver mass in mice. Biochem Biophys Rep 28:101186 (2021). PubMed: 34977363
- Castellarin M et al. A rational mouse model to detect on-target, off-tumor CAR T cell toxicity. JCI Insight 5:N/A (2020). PubMed: 32544101
- Wilson AL et al. Autoantibodies against HSF1 and CCDC155 as Biomarkers of Early-Stage, High-Grade Serous Ovarian Cancer. Cancer Epidemiol Biomarkers Prev 27:183-192 (2018). PubMed: 29141850