Goat Anti-Human IgG Fc (ab97221)
Key features and details
- Goat Anti-Human IgG Fc
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Related conjugates and formulations
Overview
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Product name
Goat Anti-Human IgG Fc
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. This antibody may cross react with IgG from other species.
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Tested applications
Suitable for: IHC-P, ELISA, WB, ICCmore details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/500. for coating; 1/1000 - 1/30000 as primary
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WB |
1/1000 - 1/10000. Colorimetric; 1/1000 - 1/30000 Chemiluminescent
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ICC |
Use at an assay dependent dilution.
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Notes |
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IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/500. for coating; 1/1000 - 1/30000 as primary |
WB
1/1000 - 1/10000. Colorimetric; 1/1000 - 1/30000 Chemiluminescent |
ICC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab97221 has been referenced in 13 publications.
- Habeichi NJ et al. Sexual dimorphism in acute myocardial infarction-induced acute kidney injury: cardiorenal deteriorating effects of ovariectomy in premenopausal female mice. Clin Sci (Lond) 137:47-63 (2023). PubMed: 36519413
- Parikh SD et al. Bioinspired Hierarchical Carbon Structures as Potential Scaffolds for Wound Healing and Tissue Regeneration Applications. Nanomaterials (Basel) 13:N/A (2023). PubMed: 37299693
- Bulaon CJI et al. Antitumor effect of plant-produced anti-CTLA-4 monoclonal antibody in a murine model of colon cancer. Front Plant Sci 14:1149455 (2023). PubMed: 37711295
- Liu H et al. Optimal target saturation of ligand-blocking anti-GITR antibody IBI37G5 dictates FcγR-independent GITR agonism and antitumor activity. Cell Rep Med 3:100660 (2022). PubMed: 35732156
- Gao Y et al. Saponins from Panax japonicus ameliorate age-related renal fibrosis by inhibition of inflammation mediated by NF-?B and TGF-ß1/Smad signaling and suppression of oxidative stress via activation of Nrf2-ARE signaling. J Ginseng Res 45:408-419 (2021). PubMed: 34025134