Overview
Product nameGoat IgG AgaroseSee all IgG isotype controls ...
ConjugationAgarose
Tested applicationsIP
more details
General notesGoat IgG was coupled to agarose beads using a cyanogen bromide method.
Goat IgG concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0mg of IgG, before coupling to agarose beads.
Properties
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1M Sodium chloride, 0.01M PBS, pH 7.2
Concentration information loading...
Purification notesGoat IgG was coupled to agarose beads using a cyanogen bromide method.
Clonality
Polyclonal
IsotypeIgG
Research Areas
RelevanceImmunoglobulin G (IgG), is one of the most abundant proteins in serum. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Cellular localizationSecreted
-
Alternative names
Ig gamma 1 chain C regionIgGIGHG1
Immunoglobin heavy constant gamma 1Immunoglobulin G
see all
Cellular localizationSecreted
Applications
Our Abpromise guarantee covers the use of
ab104155
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| IP |
IP: Use at an assay dependent dilution. Use 15-25µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.
ab104155 is an Immunoprecipitation (IP) control that is used in conjuction with agarose-immobilize antibodies made in goat. |
References for Goat IgG Agarose (ab104155)
ab104155
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"