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Rabbit F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (TRITC ), pre-adsorbed (ab5752)

Overview

  • Product nameRabbit F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (TRITC ), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesGoat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
  • Tested applicationsICC/IF, Immunomicroscopy, Flow Cyt, ELISA more details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Goat IgG whole molecule.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide, 0.01% Thimerosal.
    Constituents: 15mg/ml BSA (IgG and Protease free), 0.25M Sodium Chloride, 0.02M Potassium Phosphate, 0.01M Sodium Phosphate. pH 7.6
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesTetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Rabbit IgG F(ab’)2
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab5752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/1000 - 1/5000.
Immunomicroscopy IM: Use at an assay dependent dilution.
Flow Cyt Flow Cyt: 1/500 - 1/2500.
ELISA ELISA: 1/10000 - 1/50000.

References for Rabbit F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (TRITC ), pre-adsorbed (ab5752)

ab5752 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"