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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Donkey anti-Goat IgG H&L (PE) secondary antibody
See all IgG products (1691) ...
Description
Donkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (PE), pre-adsorbed
Target species
Goat
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins or other Goat heavy or light chain proteins.
Tested applications
ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
Minimal
cross-reactivity
Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Ratmore details
Immunogen
Goat IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Goat >> IgG >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (PE), pre-adsorbed (ab7004)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab7004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/100 - 1/250.ICC/IF: 1/100 - 1/250.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution. (As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications.)
Flow Cyt: 1/100 - 1/250.(The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. )
Donkey anti-Goat IgG H&L (PE) secondary antibody images:
Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (PE), pre-adsorbed (ab7004)
Ab7004 was used at dilution 1/50 with the primary antibody ab40821 in Flow Cyt. See the review on ab40821.
References for Donkey anti-Goat IgG H&L (PE) secondary antibody (ab7004)
This product has been referenced in:
- Miles Aet al. CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow. Am J Physiol Gastrointest Liver Physiol 294:G1257-67 (2008).Read more (PubMed: 18308860) »
See 1 publication for this product
Publishing research using ab7004? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"