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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Rabbit anti-Goat IgG H&L (Biotin) secondary antibody
See all IgG products (1691) ...
Description
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (Biotin)
Target species
Goat
Tested applications
Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
Immunogen
Goat IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Goat >> IgG >> Biotin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot Blot
ELISA
IHC-Fr
Immunomicroscopy
ICC/IF
WB
Application notes
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IHC-P: 1/100 (PMID 18930986).
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Rabbit anti-Goat IgG H&L (Biotin) secondary antibody (ab6740)
This product has been referenced in:
- Mühlmann Get al. TROP2 expression as prognostic marker for gastric carcinoma. J Clin Pathol 62:152-8 (2009). IHC-P.Read more (PubMed: 18930986) »
- Zhang Net al. Targeted and untargeted CD137L fusion proteins for the immunotherapy of experimental solid tumors. Clin Cancer Res 13:2758-67 (2007).Read more (PubMed: 17460060) »
See all 3 publications for this product
Publishing research using ab6740? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"