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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Rabbit anti-Goat IgG H&L (AP) secondary antibody
See all IgG products (1691) ...
Description
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP)
Target species
Goat
Tested applications
Dot Blot, ELISA, ICC/IF, WB, IHC-P, IHC-Frmore details
Immunogen
Goat IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Goat >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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ELISA - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab6742)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent concentration.
ELISA: 1/5000
ICC/IF
ICC/IF: 1/200 - 1/1000.ICC/IF: 1/200 - 1/1000.
WB
WB: 1/1000 - 1/4000.WB: 1/1000 - 1/4000.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
Rabbit anti-Goat IgG H&L (AP) secondary antibody images:
ELISA - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab6742)
Ab6742 was used at dilution 1/4000 with the primary antibody ab20773 in ELISA. See the review on ab20773.
References for Rabbit anti-Goat IgG H&L (AP) secondary antibody (ab6742)
This product has been referenced in:
- Takayama Het al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA.Read more (PubMed: 18523555) »
See 1 publication for this product
Publishing research using ab6742? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"