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Product Name
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Goat IgG secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Donkey polyclonal to Goat IgG - H&L (FITC)
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Immunogen
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Full length native Goat IgG (purified).
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IM
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Cellular localization
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Secreted
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Research areas
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Secondary antibodies >> anti-Goat >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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Raised in
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Donkey
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Form
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Liquid
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Conjugation
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Fluorescein
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Goat IgG secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Goat IgG secondary antibody - H&L (ab6881)
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This product has been used in: (two most recent references)
Bocksteins E et al. Kv2.1 and silent Kv subunits underlie the delayed rectifier K+ current in cultured small mouse DRG neurons. Am J Physiol Cell Physiol 296:C1271-8 (2009). ICC/IF. PubMed: 19357235
Miranda E et al. The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB. Hum Mol Genet 17:1527-39 (2008). ICC/IF. PubMed: 18267959
See all 3 publication references for this product.
If you publish research using ab6881 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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