Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3.5 ®), pre-adsorbed (ab6950)
Overview
- Product nameDonkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3.5 ®), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesGoat
- Tested applicationsICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA more details
-
Minimal
cross-reactivity
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details - Immunogen
Full length native Goat IgG (purified).
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
- Conjugation notesCy3.5 (Cyanine 3.5-OSu) (Molecular Weight 1286 daltons) Absorption Wavelength: 581 nm Emission Wavelength: 596 nm Fluorochrome/Protein Ratio: 6.9 moles Cy3.5 per mole of Donkey IgG
- Clonality Polyclonal
- General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
This material is also subject to proprietary rights of GE Healthcare
Bio-Sciences Corp. and Carnegie Mellon University and made and
sold under License from GE Healthcare Bio-Sciences Corp. This
product is licensed for sale only for research. It is NOT licensed for
any other use. There is no implied license hereunder for any
commercial use.
COMMERCIAL USE shall include:
1 Sale, lease, license or other transfer of the material or any material
derived or produced from it.
2 Sale, lease, license or other grant of rights to use this material or any
material derived or produced from it.
3 Use of this material to perform services for a fee for third parties.
If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | |
| IHC-P | |
| IHC-Fr | |
| ICC | |
| Flow Cyt | |
| ELISA |
ICC: Use at an assay dependant concentration.
IF: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3.5 ®), pre-adsorbed (ab6950)
ab6950 has not yet been referenced specifically in any publications.
