Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/2500.
1/100 - 1/500.
1/10000 - 1/200000. (primary).
1/10000 - 1/200000. (chemiluminescent).
This product has been referenced in:
Emgård J et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity48:120-132.e8 (2018).
Read more (PubMed: 29343433) »
Ji YC et al. Extraventricular neurocytoma in the left temporal lobe: A case report and review of the literature. Oncol Lett11:3579-3582 (2016).
Read more (PubMed: 27313678) »