Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab97037)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Overview
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Product name
Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details -
Conjugation
Cy5 ®. Ex: 650nm, Em: 667nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/500.
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/100 - 1/1000.
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Notes |
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IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/100 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (12)
ab97037 has been referenced in 12 publications.
- Pallares-Rusiñol A et al. Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes. Anal Chem 95:2487-2495 (2023). PubMed: 36683335
- Bernuz M et al. Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications. Methods Mol Biol 2668:257-276 (2023). PubMed: 37140802
- Moura SL et al. The activity of alkaline phosphatase in breast cancer exosomes simplifies the biosensing design. Biosens Bioelectron 198:113826 (2022). PubMed: 34891059
- Anacker A et al. Purification of Fibroblasts From the Spiral Ganglion. Front Neurol 13:877342 (2022). PubMed: 35493807
- Akkermans O et al. GPC3-Unc5 receptor complex structure and role in cell migration. Cell 185:3931-3949.e26 (2022). PubMed: 36240740