Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Mouse IgG, whole molecule (H&L)
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Conjugation
Cy5 ®. Ex: 650nm, Em: 667nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
Conjugation notes
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 12.6 moles Cy5 per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species (human labelled with the fluorophore Cy5 here) in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/10000 - 1/50000. PubMed: 18691532
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ICC/IF | (1) |
1/1000 - 1/5000.
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Flow Cyt |
Use at an assay dependent dilution.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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ELISA
1/10000 - 1/50000. PubMed: 18691532 |
ICC/IF
1/1000 - 1/5000. |
Flow Cyt
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Images
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Formation of TT3s between LSCC cells.
(Panel G) TT4s form between the intersecting rear or secondary lamellipodia establishing functional Cx43 GJs (the arrow in the inset) as confirmed by patch-clamp measurements.Cells were grown in 24-well plates with glass coverslips on the bottom, fixed with 4% paraformaldehyde for 15 min, and permeabilized with 0.2% Triton X-100/PBS for 3 min. Coverslips were incubated for 1 h with the following primary antibodies: mouse anti-α-tubulin, rabbit anti-Cx43, mouse anti-Cx43, rabbit anti-Cx26, rabbit anti-Cx30, then rinsed with 1% BSA/PBS and incubated with secondary goat anti-mouse IgG H&L (Cy5) (ab6563, Abcam Cambridge, UK) or with donkey anti-rabbit IgG (FITC) for 30 min. The F-actin network was visualized using Alexa Fluor 594 phalloidin; coverslips were incubated with the dye for 30 min at 37°C. Coverglasses were attached using Vectashield Mounting Medium with DAPI and sealed with clear nail polish. MitoTracker Green was used to stain mitochondria in live cells.
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ab2792 (PDI antibody (RL90) - ER marker) staining human U2-OS (bone osteosarcoma) cells by ICC/IF. ab6563 (undiluted) was used as the secondary.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (62)
ab6563 has been referenced in 62 publications.
- Shafique S et al. Gestational exposure to triphenyl phosphate induces epigenetic modifications in C57Bl/6 fetal liver. Birth Defects Res 115:338-347 (2023). PubMed: 36369707
- Schönherr-Hellec S et al. Implantation of engineered human microvasculature to study human infectious diseases in mouse models. iScience 26:106286 (2023). PubMed: 36942053
- Hayashi Y et al. Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity. iScience 25:104547 (2022). PubMed: 35754738
- Hu Y et al. RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss. EMBO Rep 22:e52481 (2021). PubMed: 34121311
- Sun W et al. Safety, Immunogenicity, and Protective Efficacy of an H5N1 Chimeric Cold-Adapted Attenuated Virus Vaccine in a Mouse Model. Viruses 13:N/A (2021). PubMed: 34960689