This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab128874 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773. Loading control to GAPDH ab8245 antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green Anti-Brd4 antibody [EPR5150(2)] ab128874 observed at 150 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW)-ab216773 as secondary antibody. Red - Anti-GAPDH antibody loading control, ab8245, observed at 37 kDa,
Other - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
has not yet been referenced specifically in any publications.
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