Goat Anti-Mouse IgG2a heavy chain (HRP) preadsorbed (ab98698)
Key features and details
- Goat Anti-Mouse IgG2a heavy chain (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Mouse IgG2a heavy chain (HRP) preadsorbed
See all IgG2a secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG2a was detected. This antibody may cross react with IgG2a from other species. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Human, Rat more details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG2a was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use at an assay dependent dilution.
|
|
IHC-P |
1/200 - 1/2000.
|
|
ELISA |
1/10000 - 1/100000. Primary
|
|
WB |
1/2000 - 1/20000. Colorimetric
1/5000 - 1/25000 Chemiluminescent
|
Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. Primary |
WB
1/2000 - 1/20000. Colorimetric 1/5000 - 1/25000 Chemiluminescent |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab98698 has been referenced in 9 publications.
- Wang W et al. Development of a skin- and neuro-attenuated live vaccine for varicella. Nat Commun 13:824 (2022). PubMed: 35149692
- Wu F et al. Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity. Acta Pharm Sin B N/A:N/A (2022). PubMed: 35846427
- Nardo D et al. In vivo assessment of triazine lipid nanoparticles as transfection agents for plasmid DNA. Biomater Sci 10:6968-6979 (2022). PubMed: 36222485
- Cruz-Resendiz A et al. An ambient-temperature stable nanoparticle-based vaccine for nasal application that confers long-lasting immunogenicity to carried antigens. Front Immunol 13:1057499 (2022). PubMed: 36389760
- Blokhina EA et al. Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin. Plants (Basel) 9:N/A (2020). PubMed: 32013187
- Shu Z et al. Viridicatol Isolated from Deep-Sea PenicilliumGriseofulvum Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation. Mar Drugs 18:N/A (2020). PubMed: 33081290
- Cruz-Reséndiz A et al. A self-aggregating peptide: implications for the development of thermostable vaccine candidates. BMC Biotechnol 20:1 (2020). PubMed: 31959159
- Ito R et al. Activation of adiponectin receptors has negative impact on muscle mass in C2C12 myotubes and fast-type mouse skeletal muscle. PLoS One 13:e0205645 (2018). PubMed: 30308063
- Norton TS et al. The Aspergillus fumigatus farnesyltransferase ß-subunit, RamA, mediates growth, virulence, and antifungal susceptibility. Virulence 8:1401-1416 (2017). WB . PubMed: 28489963