Goat Anti-Mouse IgG2b heavy chain (HRP) preadsorbed (ab98703)
Key features and details
- Goat Anti-Mouse IgG2b heavy chain (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Overview
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Product name
Goat Anti-Mouse IgG2b heavy chain (HRP) preadsorbed
See all IgG2b secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: IHC-P, ELISA, WB, ICCmore details -
Minimal
cross-reactivity
Human, Rat more details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/10000 - 1/100000. (Primary EISA).
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WB |
1/2000 - 1/25000. (Colorimetric: 1/2000-1/20000 dilution, Chemiluminescent: Use 1/5000-1/25000 dilution).
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|
ICC |
Use at an assay dependent dilution.
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Notes |
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IHC-P
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. (Primary EISA). |
WB
1/2000 - 1/25000. (Colorimetric: 1/2000-1/20000 dilution, Chemiluminescent: Use 1/5000-1/25000 dilution). |
ICC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab98703 has been referenced in 3 publications.
- Tong Y et al. Tubeimuside I improves the efficacy of a therapeutic Fusobacterium nucleatum dendritic cell-based vaccine against colorectal cancer. Front Immunol 14:1154818 (2023). PubMed: 37207216
- Nardo D et al. In vivo assessment of triazine lipid nanoparticles as transfection agents for plasmid DNA. Biomater Sci 10:6968-6979 (2022). PubMed: 36222485
- Pitts MG et al. Autoantibody Responses to Apolipoprotein A-I Are Not Diet- or Sex-Linked in C57BL/6 Mice. Immunohorizons 4:455-463 (2020). PubMed: 32759326