Goat Anti-Mouse IgG3 heavy chain (HRP) (ab97260)
Key features and details
- Goat Anti-Mouse IgG3 heavy chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Mouse IgG3 heavy chain (HRP)
See all IgG3 secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity with other immunoglobulins is less than 2%. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/10000 - 1/100000. (as Primary)
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WB |
1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. (as Primary) |
WB
1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab97260 has been referenced in 20 publications.
- Chen S et al. Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines. Small 19:e2205819 (2023). PubMed: 36564365
- Jia JX et al. A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching. J Nanobiotechnology 21:175 (2023). PubMed: 37264420
- Chuang YM et al. A mosquito AgTRIO mRNA vaccine contributes to immunity against malaria. NPJ Vaccines 8:88 (2023). PubMed: 37286568
- Kovalenko AO et al. Vaccine Candidate Against COVID-19 Based on Structurally Modified Plant Virus as an Adjuvant. Front Microbiol 13:845316 (2022). PubMed: 35295298
- Kong XW et al. Vesicular Stomatitis Virus-Based Epstein-Barr Virus Vaccines Elicit Strong Protective Immune Responses. J Virol 96:e0033622 (2022). PubMed: 35404082