Goat Anti-Mouse IgG+IgM+IgA H&L (HRP) (ab102448)
Key features and details
- Goat Anti-Mouse IgG+IgM+IgA H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG+IgM+IgA H&L (HRP)
See all IgG + IgM + IgA secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts with mouse IgG, IgA and IgM. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with immunoglobulins from other species. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/200 - 1/5000.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. Primary dilution.
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WB |
1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution.
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Notes |
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ICC
1/200 - 1/5000. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. Primary dilution. |
WB
1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab102448 has been referenced in 7 publications.
- Jiang Y et al. Periostin regulates LPS-induced apoptosis via Nrf2/HO-1 pathway in periodontal ligament fibroblasts. Oral Dis 29:2188-2204 (2023). PubMed: 35298860
- Kou Y et al. Eldecalcitol prevented OVX-induced osteoporosis through inhibiting BMSCs senescence by regulating the SIRT1-Nrf2 signal. Front Pharmacol 14:1067085 (2023). PubMed: 36937895
- Jung H et al. Atg14 protects the intestinal epithelium from TNF-triggered villus atrophy. Autophagy 15:1990-2001 (2019). PubMed: 30894050
- Luo T et al. Adipocytes enhance expression of osteoclast adhesion-related molecules through the CXCL12/CXCR4 signalling pathway. Cell Prolif 50:N/A (2017). PubMed: 27868262
- Liu B et al. Immunolocalization of MMP9 and MMP2 in osteolytic metastasis originating from MDA-MB-231 human breast cancer cells. Mol Med Rep 14:1099-106 (2016). IHC-P . PubMed: 27278284
- Hagarman JA et al. Coordinate regulation of DNA methylation and H3K27me3 in mouse embryonic stem cells. PLoS One 8:e53880 (2013). PubMed: 23326524
- Murphy PJ et al. Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells. Proc Natl Acad Sci U S A 110:7772-7 (2013). WB . PubMed: 23610441