Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality Polyclonal
  • General notes

    The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab175652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

from abreview 42056

ELISA Use at an assay dependent concentration.
Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.

A dedicated 405 filter set is very advantageous. DAPI filter sets work to a point, but they excite on the lower of 405’s 2 excitation peaks, where a dedicated 405 filter set will excite on the higher peak. Depending on the characteristics of the particular DAPI emission filter being used, it may or may not encapsulate the maximum emission peak of 405. A brighter signal will therefore be seen observing 405 under a dedicated filter set, rather than one optimised for DAPI.

Ex max: 405 = 402nm / DAPI = 359nm

Em max: 405 = 421nm / DAPI = 461nm

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) images

  • The cells were 100% methanol fixed (5 min) and then
    incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) and (ab37266, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Green LCS1 (ab138904) was used to stain the cell nuclei (green) at a dilution of 1/500.

  • ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.

References for Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

ab175652 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunohistochemistry (Frozen sections)
Rabbit antibodies raised against coccaine- and amphetamine-regulated transcipt (CART) were visualised with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) and viewed under an epifluorescent micropsope eqipped with UV filter.
Username

Prof. Marcin Arciszewski

Verified customer

Submitted Mar 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"