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I would like to perform some study about Immunofluorescence staining of lysosome. I am wondering if the primary antibody and secondary antibody that I selected are correct. Primary antibody (abcam): Anti-LAMP1 antibody (ab24170); Secondary antibody (Invitrogen): Alexa Fluor(tm); 488 Goat Anti-Rabbit IgG (H+L) (ab150077).
ps. What's the difference between "Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor(tm) 488) (ab150073 and Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor(tm); 488) (ab150077) for lysosomal staining?
Asked on Feb 26 2013
The LAMP-1 antibody ab24170 is an excellent choice for staining this lysosome marker, and the anti- rabbit IgG ab150077 is the correct secondary antibody.
The antibody raised in donkey, ab150073, would also work, and as far as we know there is no advantage to using one or the other. However, if your sample contains endogenous IgG, you may want to consider using a secondary antibody that has been purified to remove reactivity with IgG of species other than rabbit. I suggest ab150085: http://www.abcam.com/index.html?datasheet=150085 .
Abcam Scientific Support
Answered on Feb 26 2013