Detection of Tet2 (with ab124297 Primary) in D3 mouse Embryonic Stem cells using ab150077 - Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) as Secondary Antibody.

Immunocytochemistry/ Immunofluorescence
Cells were fixed in 4% PFA, permeabilized using 0.1% Triton X-100, blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes at RT then incubated with ab124297 at a 1/100 dilution or Rabbit IgG Isotype control for 2 hours at RT. The secondary used was a Goat polyclonal Secondary Antibody to Rabbit IgG – H&L Alexa Fluor 488 (ab150077) used at 1/200 dilution.

Joe Segal

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Submitted Aug 16 2013

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