Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab7091)
Key features and details
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Dot blot, IHC-Fr
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, Dot blot, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200 - 1/1000.
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IHC-P |
Use at an assay dependent dilution.
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WB |
1/500 - 1/2500.
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ELISA |
1/8000.
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Dot blot |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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Notes |
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ICC/IF
1/200 - 1/1000. |
IHC-P
Use at an assay dependent dilution. |
WB
1/500 - 1/2500. |
ELISA
1/8000. |
Dot blot
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab7091 has been referenced in 4 publications.
- Ma X et al. Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma. Cancer Cell Int 21:246 (2021). PubMed: 33941190
- Svobodová Kovaríková A et al. N6-Adenosine Methylation in RNA and a Reduced m3G/TMG Level in Non-Coding RNAs Appear at Microirradiation-Induced DNA Lesions. Cells 9:N/A (2020). PubMed: 32033081
- Svobodová Kovaríková A et al. Spatiotemporal Mislocalization of Nuclear Membrane-Associated Proteins in ?-Irradiation-Induced Senescent Cells. Cells 9:N/A (2020). PubMed: 32316379
- Svobodová Kovaríková A et al. H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions. Aging (Albany NY) 10:2585-2605 (2018). PubMed: 30312172