Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)
Key features and details
- Goat Anti-Rabbit IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody reactivity was detected against non-immunoglobulin serum proteins.
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Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Conjugation notes
Biotin/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/500 - 1/5000.
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ICC/IF |
1/200 - 1/1000.
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ELISA |
1/20000 - 1/400000. ELISA-Primary
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WB |
1/20000 - 1/400000. WB-Chemiluminescent
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Notes |
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IHC-P
1/500 - 1/5000. |
ICC/IF
1/200 - 1/1000. |
ELISA
1/20000 - 1/400000. ELISA-Primary |
WB
1/20000 - 1/400000. WB-Chemiluminescent |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab97049 has been referenced in 27 publications.
- Friedhoff J et al. BAP1 and PTEN mutations shape the immunological landscape of clear cell renal cell carcinoma and reveal the intertumoral heterogeneity of T cell suppression: a proof-of-concept study. Cancer Immunol Immunother 72:1603-1618 (2023). PubMed: 36562826
- Agas A et al. Hemolytic iron regulation in traumatic brain injury and alcohol use. Alcohol 109:1-12 (2023). PubMed: 36690222
- Hososhima S et al. Proton-transporting heliorhodopsins from marine giant viruses. Elife 11:N/A (2022). PubMed: 36065640
- Ferdousi MI et al. Hyporesponsivity to mu-opioid receptor agonism in the Wistar-Kyoto rat model of altered nociceptive responding associated with negative affective state. Pain 162:405-420 (2021). PubMed: 32826755
- Moudi B et al. Cannabinoid receptor type-1 and its correlation with CB1 gene polymorphism-1359G/A in ectopic pregnancy compared to the control group. J Obstet Gynaecol Res 47:1256-1264 (2021). PubMed: 33511667