Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
- Datasheet
- Specific References (14)
- Protocols
Overview
- Product nameGoat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
See all IgG secondary antibodies - Target speciesRabbit
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
- Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
- Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details - ConjugationDyLight® 488. Ex: 493nm, Em: 518nm
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Applications
Our Abpromise guarantee covers the use of ab96899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/500. | |
| ICC/IF | 1/50 - 1/500. | |
| Flow Cyt | 1/500. |
Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed images
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Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)ICC/IF image of (ab3280) stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3280, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
DyLight® - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
References for Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
This product has been referenced in:
- Naylor RW et al. Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish. Sci Rep 6:35647 (2016). Read more (PubMed: 27759103) »
- Liegel RP et al. Alkylglycerone phosphate synthase (AGPS) deficient mice: models for rhizomelic chondrodysplasia punctate type 3 (RCDP3) malformation syndrome. Mol Genet Metab Rep 1:299-311 (2014). Read more (PubMed: 25197626) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"