Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)

Submit an Abreview Q&A (13)Specific References (15)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/500.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)
    Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)
    ICC/IF image of (ab3280) stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3280, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • DyLight® - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)
    DyLight® - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Bai Y  et al. Atractylodin Induces Myosin Light Chain Phosphorylation and Promotes Gastric Emptying through Ghrelin Receptor. Evid Based Complement Alternat Med 2017:2186798 (2017). Read more (PubMed: 28883883) »
  • Naylor RW  et al. Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish. Sci Rep 6:35647 (2016). Read more (PubMed: 27759103) »

See all 15 Publications for this product

Publishing research using ab96899? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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13 reviews or Q&A

10
Votes: Highest First

Bonjour,

Je travaille sur des cellules équines et je suis à la recherche d’un anticorps anti-Mucin5AC qui ne soit pas mouse.

Je viens de voir sur votre site que vous aviez un anticorps rabbit anti-mucin5AC (ab7866...

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Merci de votre intérêt pour l'anti-Mucin5AC ab78660 et le secondaire ab96899.

En effet, l'anticorps ab78660 n’a pas encore été testé chez le cheval et nous n’offrons pas d’échantillon ...

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Customer kindly contacted us to inquire about the immunogen sequence for ab56215.

Thank you for contacting us and your interest in our products. The immunogen sequence used to raise theab56215 Anti-SLC4A4 antibodyis proprietary information. However, in order to aid your research I am able to share it with you. Please do not share...

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Since neither ab28856 nor ab51059 is tested in IHC-Fr. We are thinking of testing ab28856 first.

Could you send me a discount code for testing ab28856 (Anti-NFkB p65 (phospho S536) antibody) only?

DISCOUNT CODE: **********
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab28856 inIHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this...

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Testing discount info for ab28856 & ab51059 in IHC-Fr sections.

Thank you very much for your interest in 28856 & ab51059.

To our knowledge, 28856 & ab51059 have not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy 28856 & ab51059 now, test it in IHC-Fr...

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Thank you for your reply. I checked this product and it says that the target species is Rabbit while I want to apply it on Human tissue. so does it work on human tissue? if not can you please suggest another appropriate product.



...

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Thank you for contacting us.

The ab292 is a rabbit antibody which target human collagen I. To detect this primary you would need secondary which is ab96899.

If your primary ab works in human then the secondary ab will work.
<...

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Inquiry: I ordered an anti-collagen type1 from Abcam (Rabbit poly, ab292) and I want to apply Immunofluorescence experiment on HUMAN cornea tissue. I need to order now an anti mouse/rabbit? secondary antibody conjugated with AlexaFluor 488. So, first i...

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Thank you for contacting us.

Please buy ab96899;http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96899.html

The Dylight 488 conjugate is better than Alexa 488.

I hope t...

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Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early respo...

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Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please e...

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Thank you very much! In this way I should be able to image the aggrecan in green and the collagen II in red, is this correct? Is there any kind of discount or special price for this package?

It is my pleasure to help.

In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttp://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those col...

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Hello, I need to image collagen type II and aggrecan from mouse within the same samples. The laser that I have available are 458, 488, 514, 543, 633. Which combination of antibodies (primary and secondary) do you recommend that might work nicely t...

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Thank you for contacting us.

We presently have only 1 product against mouse aggrecan, ab36861. This is a rabbit polyclonal. In ICC and appropriate secondary would be anti-Rabbit IgG with a fluorescent tag which could be excited by your las...

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I have run an IF-staining on bronchoalveolar cells (human: mainly alveolar macrophages and T lymphocytes; cytospin slides 60 000 cell per slide; sarcoidosis patients) with:

1- Anti-PPAR alpha antibody (ab61182) + Goat polyclonal Secondary An...

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Thank you for contacting us.
To answer your questions:
1. Yes, a negative control can be ommiting the primary. Other options could be using an isotype control antibody that compares with the priamry, or the blocking peptide. There are various...

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