Blocking buffer - 5%BSA/1%OvAlb/PBS for 3 hours.
Primary antibody - NR1D1(ab174309) 1:1000, 15 hours at 4C, expected size 67kDa.
Secondary diluted in TBST (1:5000) for 3 hours at room temperature.
ECL prime: 1 second exposure.
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Submitted Aug 12 2016