Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)

Overview

  • Product name
    Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
  • Target species
    Rabbit
  • Specificity
    This antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
  • Tested applications
    Suitable for: WB, In-Cell ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length protein corresponding to Rabbit IgG.

  • Conjugation
    IRDye® 800CW

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    pH: 7.4
    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Affinity chromatography purified using pooled rabbit IgG covalently linked to agarose.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/25000.
In-Cell ELISA 1/200 - 1/1200.

Images

  • All lanes : Anti-NF-kB p65 antibody (ab16502) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : NF-kB p65 knockout HAP1 cell lysate (20 µg)
    Lane 3 : HeLa cell lysate (20 µg)
    Lane 4 : A431 cell lysate (20 µg)

    Secondary
    Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16502 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Merged signal (red and green). Green - ab16502 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.

  • All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml (Anti-Lamin B1 antibody - Nuclear Envelope Marker)

    Lane 1 : Wild-type HAP1 nuclear lysate, 10 ug
    Lane 2 : Lamin B1 knockout HAP1 nuclear lysate

    Secondary
    Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16048 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Merged signal (red and green).  Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 ( (loading control to histone H3 at 0.1µg/ml) observed at 18 kDa using as secondary Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.

  • Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.

References

ab216773 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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