Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)
Overview
- Product nameGoat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin)See all IgY secondary antibodies ...
- Target speciesChicken
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Tested applicationsELISA, IHC-P, ICC/IF, WB more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
- Conjugation notesBiotin/ antibody protein ratio is 4:1
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab97133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/20000 - 1/400000. (Primary). |
| IHC-P | IHC-P: 1/500 - 1/5000. |
| ICC/IF | ICC/IF: 1/200 - 1/1000. |
| WB | WB: 1/20000 - 1/400000. (Chemiluminescent). |
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)Ab97133 was used at dilution 1/200 with the primary antibody ab81755 in IHC-P. See the review on ab81755.
References for Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)
ab97133 has not yet been referenced specifically in any publications.
