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Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin), pre-adsorbed (ab7117)

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Overview

  • Product nameGoat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin), pre-adsorbedSee all IgY secondary antibodies ...
  • Target speciesChicken
  • Tested applicationsICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB more details
  • Minimal
    cross-reactivity
    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native chicken IgY (purified).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgY fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
Dot Blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • Application notesDot: Use at an assay dependant dilution.
    ELISA: 1/8000 - 1/36000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin), pre-adsorbed (ab7117)

    ab7117 has not yet been referenced specifically in any publications.

    Publishing research using ab7117 ? Please let us know so that we can cite the reference in this datasheet

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    Thanks for your help, but I am still uncertain about my question regarding the addition of glycerol. "The storage instructions stated to add 50% glycerol before freezing. Should this be 50% of the current volume or a final concentration of 50%".

    Thank you for your message. I have double-checked with my colleagues and actually we have reconstituted this antibody and already added 50% glycerol to the buffer. This means that for long term storage, you do not need to add anything just aliquot the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"