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Goat anti-Chicken IgY H&L (Cy3 ®) secondary antibody (ab97145)

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Overview

  • Product nameGoat anti-Chicken IgY H&L (Cy3 ®) secondary antibodySee all IgY secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Cy3 ®), pre-adsorbed
  • Target speciesChicken
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
  • Minimal
    cross-reactivity
    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/100 - 1/1000.

References for Goat anti-Chicken IgY H&L (Cy3 ®) secondary antibody (ab97145)

ab97145 has not yet been referenced specifically in any publications.

Publishing research using ab97145 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab97145 Submit a Question for ab97145

Displaying 1 - 2 of 2 results for Abreviews and Q&A

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Votes: Highest First

Auto-fluorescence in the tissue. Sudan black treatment gave tissue a red tint.



As we discussed, please send me the images of the staining. I would suggest using a positive control and confirming the GFP transfection, but trying a fluorophore from an infrared channel where the auto-fluorescence is lower may be a good soluti...

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Customer kindly contacted us to discuss use of this product in IHC-P, issues with autofluorescence and secondary antibody choices.

Thank you for contacting us.

I have included with this email 2 documents about autofluorescence and tips for reducing this as requested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"