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Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed (ab96954)

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Overview

  • Product nameGoat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbedSee all IgY secondary antibodies ...
  • Target speciesChicken
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
  • Minimal
    cross-reactivity
    Cow, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 650 replaces DyLight® 649.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed images

  • DyLight®-Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed(ab96954)
    DyLight®-Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed(ab96954)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed (ab96954)

ab96954 has not yet been referenced specifically in any publications.

Publishing research using ab96954 ? Please let us know so that we can cite the reference in this datasheet

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How many DyLight 650 fluorophore molecules are conjugated to each IgG molecule?

Thank you for your inquiry.


I heard back from the lab that the ratio of DyLight650 to Protein (F/P)varies per lot for ab96954.

Lot GR66644-3: F/P = 5.5

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"