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Goat polyclonal Secondary Antibody to Human IgG - Fc (DyLight® 550) (ab97004)

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Overview

  • Product nameGoat polyclonal Secondary Antibody to Human IgG - Fc (DyLight® 550)See all IgG secondary antibodies ...
  • Target speciesHuman
  • SpecificityThe antibody is specific to Fc region of human IgG. The Dylight conjugate is conjugated at Fc region of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Goat polyclonal Secondary Antibody to Human IgG - Fc (DyLight® 550) images

  • DyLight®-Goat polyclonal Secondary Antibody to Human IgG - Fc (DyLight® 550)(ab97004)
    DyLight®-Goat polyclonal Secondary Antibody to Human IgG - Fc (DyLight® 550)(ab97004)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat polyclonal Secondary Antibody to Human IgG - Fc (DyLight® 550) (ab97004)

ab97004 has not yet been referenced specifically in any publications.

Publishing research using ab97004 ? Please let us know so that we can cite the reference in this datasheet

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Customer asked if the goat anti human IgG is specific to the Fc region of antibody.

Thank you for contacting us. The antibody is indeed specific to the Fc region of human IgG and the Dylight conjugate is conjugated to the Fc region of antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"