You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Goat polyclonal Secondary Antibody to Mouse IgG - Fc (FITC) (ab97264)

Submit an Abreview Q&A (5)Specific References (1)

Overview

  • Product nameGoat polyclonal Secondary Antibody to Mouse IgG - Fc (FITC)See all IgG secondary antibodies ...
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notesF/P ratio is 4.4
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Goat polyclonal Secondary Antibody to Mouse IgG - Fc (FITC) images

  • Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - Fc (FITC) (ab97264)
    Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - Fc (FITC) (ab97264)Jorge E. Gonzalez (CPHR, La Habana, Cuba), Joan F. Barquinero(UAB, Barcelona,Spain) and Jessica Martinez (UAB, Universitat Autònoma de Barcelona, Spain).
    A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures.
    Primary anitibody H2A.X (phospho S139) antibody [3F2], ab22551, 100ug.
    Secondary antibody Mouse IgG-Fc (FITC), ab97264, 1mg.

References for Goat polyclonal Secondary Antibody to Mouse IgG - Fc (FITC) (ab97264)

This product has been referenced in:
  • González JE  et al. Radiosensitization induced by the anti-epidermal growth factor receptor monoclonal antibodies cetuximab and nimotuzumab in A431 cells. Cancer Biol Ther 13:71-6 (2012). ICC/IF ; Human . Read more (PubMed: 22231391) »

See 1 Publication for this product

Publishing research using ab97264 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab97264 Submit a Question for ab97264

Displaying 1 - 5 of 5 results for Abreviews and Q&A

10
Votes: Highest First

Thanks for you helpfull   The purchase order information is 902004, Date 21, june 2011.  I attach the order.   If you need other information to free feel contact to me.

Thanks for sending the purchase order information. I have located the order for ab97264. Would you like me to send another vial of this same product as the replacement?

Dear Abcam   The customer will like change the antibody ab97264 by ab6724. Attach information

Thanks for the reply. So I can certainly set up the replacement to send one vial of ab6724. For some reason the purchase order information did not make it through. If you could, please re-send this and I'll be happy to set up the replacement order....

Read More

Thanks for your assistance. Effectively, the Customer assayed to a dilution at 1/200, but she still  obtained high level of background. I will appreciate you may confirm the replacement the antibody as soon as possible, because to inform to the customer. 

Since the antibody is not working as expected I would be happy to send a replacement. I just need a bit more information to process your request: 1. What antibody would you like as the replacement? 2. What is the order number used to purchase ab...

Read More

Thanks for your reply. I have a question about your recommendation. According to the reported by the customer, it could exist the possibility the antibody is unspecific. If this situation can be, what do you suggest to check it?. I think in this case, Abc...

Read More

You are correct that if we find the antibody is not peforming as expected, based on information on our online datasheet, we would be happy to offer a replacement, credit or refund. In this case, the antibody may be reacting unspecifically. Based on...

Read More

A Customer has sent me an e-mail claiming about a product bought by him. According to the information given, he acquired an antibody, ab97264 and it has been applied in flow cytometry. However, he has obtained unspecific signal, that means, he obtained po...

Read More

Thank you for your enquiry. I would recommend that the customer increase the dilition factor for this secondary antibody from 1:50 to 1:200. I believe this will reduce the background. I hope this is helpful. Please let me know if there are ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"