Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6788)

The secondary antibody ab6788 gives no signal. We have compared it to an effective anti-mouse IgG secondary, also biotinylated.

ANSWER:

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

I am interested in purchasing a secondary antibody for use in immunohistochemistry that can work with the following primary antibodies from your company (ab24590, ab7817, ab955, and ab89064). I have searched the catalog and found ab6728 and ab6788 as possible candidates for this purpose. Could you kindly suggest on which one would work better with those primary antibodies for immunohistochemistry (IHC-P)?

Thank you,

ANSWER:

Thank you for contacting Abcam.

I feel that both products are suitable for IHC-P. Both have many specific references and in-depth scientific support data right on the datasheet. They differ mainly in the species in which they were raised and in the tags used for detection. Ab6728 is an HRP conjugate whereas Ab6788 is Biotin labeled. I believe that you should choose the secondary which has an associated detection system that you are most familiar with in case you should run into any staining issues.

I hope this information is helpful. Please let me know if you have any questions.

Dear Sir/madam, Thank you very much for your kind attention. Secondary antibody that I used it is: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6788) I have used the same secondary antibody with another mouse monoclonal Antibody and it works very well. Thank you very much again with my best regards.

ANSWER:

Thank you very much again for your reply.

I appreciate the time you have spent in the laboratory and understand your concerns. As it appears to me your biotin-labeled secondary and the appropriate detection system are indeed working and it is regrettable the results have not been successful with the Progerin antibody yet. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial of ab66587.

I apologise for the inconvenience and am pleased to offer you a free of charge replacement (with either a new vial or any other primary antibody of your choice) or credit note in compensation. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Dear Sir/madam,

Thank you very much for your kind attention.

These are the answers of all question:

1- No results, I mean that I obtain no bands at all.

2- a-The protein was denatured first.

b- 5% milk

c- 1:100 dilution

d- Anti-mouse IgGBiotin secondary Abcam ab6788 Goat

Thank you very much again with my best regards.

ANSWER:

Thank you very much for confirming these details.

Before proceeding with this case, I would appreciate if you could confirm which detection system was used with the biotin-labeled secondary antibody ab6788: Have you used a HRP-conjugated anti-biotin antibody or HRP-conjugated streptavidin, and is this working for other primary antibodies in your lab?

I look forward to your reply.

Hi, Could you please tell me the molecular weight of the antibody ab6788 in either daltons or grams (IgG is supposed to be 150,000 daltons in humans. But this one is from rabbit and has other modifications to it.) Also, your online information sheet says Biotin/protein ratio as 10 - 20. Does this mean there are 10 - 20 biotin molecules per Ab? Thank you

ANSWER:

Thank you for your enquiry.

Unfortunately, the molecular weight of this product is not specifically measured, but the molecular weight of non reduced IgG is approximately 150-160 kDa. The molecular weight of biotin is ~250 Daltons.

Yes, there are 10-20 molecules of Biotin conjugated to the IgG. The ratio of 10-20 is based on the combination ratio and typical results from our standard conjugation protocol. Function and purity of intact conjugate is confirmed by Immuno-electrophoresis (with anti biotin) and ELISA against mouse IgG using a HRP conjugated streptavidin as a secondary reagent.

I hope　this information is helpful.

If there is anything else that I can help you with, please do not hesitate to contact me.