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Goat anti-Mouse IgG H&L (Chromeo™ 546) secondary antibody (ab60316)

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Overview

  • Product nameGoat anti-Mouse IgG H&L (Chromeo™ 546) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 546)
  • Target speciesMouse
  • Specificityab60316 reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applicationsICC/IF more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Conjugation notesChromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 96,800 M-1cm-1 (measured at Amax) Quantum Yield: ~ 11 % Excitation Wavelength Range: 530 to 550 nm Emission Wavelength Range: 560 to 570 nm
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab60316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/1000 - 1/2000.
Plate-based assays: 1/800 to 1/1000.

Goat anti-Mouse IgG H&L (Chromeo™ 546) secondary antibody images

  • Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 546) (ab60316)
    Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 546) (ab60316)
    EGF receptor detected in an urothel cell line by ICC/IF using ab60316 at 1/1000. Paraffin-treated tissue.
  • Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 546) (ab60316)
    Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 546) (ab60316)
    ab60316 staining a mouse monoclonal antibody to alpha Tubulin in HeLa cells by Immunocytochemistry/Immunofluorescense.

References for Goat anti-Mouse IgG H&L (Chromeo™ 546) secondary antibody (ab60316)

ab60316 has not yet been referenced specifically in any publications.

Publishing research using ab60316 ? Please let us know so that we can cite the reference in this datasheet

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Phone call requesting information on regards to testing discount using Goat anti-Mouse IgG H&L (Chromeo™ 546) secondary antibody (ab60316) in Western blotting.

Thank you for contacting us yesterday.
As discussed over the phone, I have looked into whether there might be any suitable alternative to the Goat anti-Mouse IgG H&L (Chromeo™ 546) secondary antibody (ab60316) for you. Particularly in regards to seco...

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Dear Abcam,

I am new at immunostaining. So I would like to verify my decision before going ahead and purchasing the following primary and secondary antibodies.
I am purchasing the following primary antibodies:
1. http://www.abcam.com/...

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Thank you for contacting us.
I am very happy to help you find the most suitable antibodies for your application. For that, could you please specify if you are interested in performing immunostaining in tissues or immunocytochemistry?
Please noti...

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Recently, we have bought from you the mouse monoclonal [6A7] to bax (ab5714).
As secondary antibody, we would like to use the Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 546) (ab60316).
We are working with PC12 cell...

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Thank you for contacting us.

Yes, those two antibodies, ab5714 and ab60316, are a compatible pair, and ab5714 will recognize rat Bax.

Please note that ab5714 should not be stored frozen, but only at 4oC.

I hope this inform...

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compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"