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Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed (ab97035)

Submit an Abreview Q&A (1)Specific References (3)

Overview

  • Product nameGoat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/100 - 1/1000.

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed images

  • Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed (ab97035)
    Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed (ab97035)Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
    ab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.

References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed (ab97035)

This product has been referenced in:
  • Hindle S  et al. Dopaminergic expression of the Parkinsonian gene LRRK2-G2019S leads to non-autonomous visual neurodegeneration, accelerated by increased neural demands for energy. Hum Mol Genet N/A:N/A (2013). ICC/IF . Read more (PubMed: 23396536) »
  • Kim JW  et al. New Molecular Bridge between RelA/p65 and NF-?B Target Genes via Histone Acetyltransferase TIP60 Cofactor. J Biol Chem 287:7780-91 (2012). ICC/IF . Read more (PubMed: 22249179) »

See all 3 Publications for this product

Publishing research using ab97035 ? Please let us know so that we can cite the reference in this datasheet

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Hi, Thanks for your message and providing me options. I'd like to try the CY 3 (ab97035) in WB and take part in the testing scheme. I'd be grateful if you can send me the discount code. Kind regards

Thank you for your reply.
I'm pleased to hear you would like to participate in the testing discount scheme with the Goat anti-Mouse IgG H&L (Cy3 ®) secondary antibody (ab97035). The discount code is as follows:
Discount code: xxxxxxxx
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"