Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed (ab97035)
- Product nameGoat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesMouse
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
- Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab97035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: 1/50 - 1/500.|
|ICC/IF||ICC/IF: 1/50 - 1/500.|
|Flow Cyt||Flow Cyt: 1/100 - 1/1000.|
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed images
ab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.
References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed (ab97035)
This product has been referenced in:
- Hindle S et al. Dopaminergic expression of the Parkinsonian gene LRRK2-G2019S leads to non-autonomous visual neurodegeneration, accelerated by increased neural demands for energy. Hum Mol Genet N/A:N/A (2013). ICC/IF . Read more (PubMed: 23396536) »
- Kim JW et al. New Molecular Bridge between RelA/p65 and NF-?B Target Genes via Histone Acetyltransferase TIP60 Cofactor. J Biol Chem 287:7780-91 (2012). ICC/IF . Read more (PubMed: 22249179) »