Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab96879)

Overview

• Product nameGoat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbedSee all IgG secondary antibodies ...
• Target speciesMouse
• SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
• Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
• Minimal
  cross-reactivity
  Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat
  To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
  more details

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.2% BSA, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Secondary antibodies
  • anti-Mouse
  • IgG
  • Fluorophore
  • DyLight® 488

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed images

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  DyLight® - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab96879)
  Emission spectra of DyLight® fluorochromes available in our catalog.
  Line colors represent the approximate visible colors of the wavelength maxima.
  
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  Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab96879)
  ICC/IF image of ab40084 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
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  Flow Cytometry - Anti-CD45 antibody [F10-89-4] (ab30470)
  Overlay histogram showing Jurkat cells stained with ab30470 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30470, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min) used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.