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Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022)

Submit an Abreview Q&A (5)Specific References (2)

Overview

  • Product nameGoat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)See all IgG secondary antibodies ...
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsICC/IF, IHC-P, Flow Cyt more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notesF/P ratio is 5.1
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/50 - 1/500.
IHC-P IHC-P: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) images

  • Immunohistochemistry (Frozen sections) - Goat anti-Mouse IgG H&L (FITC) secondary antibody (ab97022)
    Immunohistochemistry (Frozen sections) - Goat anti-Mouse IgG H&L (FITC) secondary antibody (ab97022)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Mouse anti-E-cadherin, 1:100
    Secondary antibody:Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022), 1:100
    Nuclei were counterstained with DAPI

References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022)

This product has been referenced in:
  • Grenier L  et al. The activation of DNA damage detection and repair responses in cleavage-stage rat embryos by a damaged paternal genome. Toxicol Sci 127:555-66 (2012). ICC/IF . Read more (PubMed: 22454429) »
  • Murooka TT  et al. HIV-infected T cells are migratory vehicles for viral dissemination. Nature 490:283-7 (2012). Read more (PubMed: 22854780) »

See all 2 Publications for this product

Publishing research using ab97022 ? Please let us know so that we can cite the reference in this datasheet

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Could you send us SDS of ab97022 and ab97039?

Thank you for contacting us.

The safety datasheets has been published. Please download these from website.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Inquiry: respected Sir, I have to use these antibodies for detection of cryptosporidium infection in cell culture by IFA. I had perfomed the experiment by fixing Cryptospordium infected MDCk cell lines with formalin for one hour. Washing three times and t...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for ICC-IF...

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Hope my e-mail will find you in good health. I would be highly glad if you could kindly provide me with the isoelectric point (pH) or pI of mouse anti F-actin antibody [NH3] and goat antimouse IgG conjugated with FITC. Thank you very much and looking forw...

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Thank you for contacting us.

Unfortunately, we have not determined the pI (isoelectric) value for this product, ab205 Anti-F-actin antibody [NH3], nor any relevant goat anti-mouse IgG conjugated FITC antibodies like ab97022 and ab97039.
<...

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Dear Sir,
The customer will order ab3778, ab3092, ab97022 for Flow cytometry application, sample is human PBMC. So,she would like to abcam guide for Flow cytometry method ( staining step?, wash step? )

Thank you for contacting us.

Unfortunately the indicated primary antibodies have not been tested with Flow Cytometry, and therefore are not covered by our Abpromise guarantee.

I would instead recommend using a different antibody agains...

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Dears,   I know those items below are from a competitor! Do we have any of those below?   Would you please send me the quote for: 1.  Alexa488 phalloidin, vial 2.  Alexa594 phalloidin, vial 3.  Coat anti mouse secondary antibody conjugated to alexa 594 4....

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Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® flu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"