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Product name
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
See all IgG products (1703) ...
Target species
Mouse
Tested applications
ICC/IF, IP, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
Immunogen
mouse IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term. Store in single use aliquots at -20°C. Avoid refreezing an aliquot. This product is stable at 4°C as an undiluted liquid. Centrifuge product prior to usage to eliminate potential protein aggregates.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Horseradish Peroxidase (HRP)
Clonality
Polyclonal
General notes
The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
Research areas
Secondary antibodies >> anti-Mouse >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/500 - 1/2500.ICC/IF: 1/500 - 1/2500.
IP: Use at an assay dependent dilution. PubMed: 17164758
Dot: Use at an assay dependent dilution.
ELISA
ELISA: 1/100000.ELISA: 1/100000.
IHC-P
IHC-P: 1/500 - 1/2500.IHC-P: 1/500 - 1/2500.
IHC-Fr
IHC-Fr: 1/500 - 1/2500.IHC-Fr: 1/500 - 1/2500.
IM: Use at an assay dependent dilution.
WB: 1/2000 - 1/10000. PubMed: 17413034
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) images:
Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
Ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.
References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
This product has been referenced in:
- Pacini S et al. Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs). PLoS One 5:e9861 (2010). WB.Read more (PubMed: 20360837) »
- Toma L et al. Irreversibly glycated LDL induce oxidative and inflammatory state in human endothelial cells; added effect of high glucose. Biochem Biophys Res Commun 390:877-82 (2009).Read more (PubMed: 19850013) »
See all 8 publications for this product
Publishing research using ab6789? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"