Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020)
Overview
- Product nameGoat polyclonal Secondary Antibody to Mouse IgG - H&L (AP)See all IgG secondary antibodies ...
- Target speciesMouse
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Tested applicationsICC, ELISA, WB, IHC-P more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
- Conjugation notesMolar enzyme: antibody protein ratio is 1:1
- Clonality Polyclonal
- IsotypeIgG
- General notesPart of the AbExcel range.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab97020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: Use at an assay dependent dilution. |
| ELISA | ELISA: 1/5000 - 1/50000. (Primary ELISA) |
| WB | WB: 1/5000 - 1/50000. (Chemiluminescent) |
| IHC-P | IHC-P: 1/100 - 1/1000. |
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) images
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Western blot - Goat anti-Mouse IgG H&L (AP) secondary antibody (ab97020)All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1 -6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/20000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020)
This product has been referenced in:
- Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). WB . Read more (PubMed: 23223527) »
