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Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) (ab97230)

Submit an Abreview Q&A (4)Specific References (1)

Overview

  • Product nameGoat polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP)See all IgM secondary antibodies ...
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsELISA, WB, ICC, IHC-P more details

Properties

Applications

Our Abpromise guarantee covers the use of ab97230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/10000 - 1/100000. (Primary ELISA)
WB WB: 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000)
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/200 - 1/2000.

References for Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) (ab97230)

This product has been referenced in:
  • Xu M  et al. Trinucleotide repeat deletion via a unique hairpin bypass by DNA polymerase ß and alternate flap cleavage by flap endonuclease 1. Nucleic Acids Res 41:1684-97 (2013). WB . Read more (PubMed: 23258707) »

See 1 Publication for this product

Publishing research using ab97230 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab97230 Submit a Question for ab97230

Displaying 1 - 4 of 4 results for Abreviews and Q&A

10
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I have some specifictechnical questions regarding your secondary antibodies:

1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

mouse mAb anti human IgG1-HR...

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Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to y...

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Dear technical team,

Our customer tests those antibodies with nano particles and there are amine group on nano particle.
The customer would like to ask if antibody and amine group can binding.
If you have information, please let me kn...

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Thank you for contacting us.

I am sorry but I do not fully understand what the customer is hoping to achieve and have thereforefound it difficult to answer your question. How are they testing the antibodies with nanoparticles?

It is un...

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Dear technical team, Our customer has ab21035(IgM), ab97230(IgG) and is going to conjugate FITC.   She wants to know that antibody has -COOH or -NH2. I know generally whole antibodies have -cooH group at the Fc region but don't know about -NH2.   ...

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Thank you for your inquiry. Indeed, antibodies as most (unmodified) proteins have a carboxy group (-COOH) at their C-terminus, as you indicated. They also have an amine group (-NH2) at their N-terminus. Furthermore, antibodies contain amino acids whic...

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 Dear technical team,   Our customer purchased ab21035 and ab97230.   She has test them with chemiliuminescent method in vial.   She incubates antigen(magnetic bead binded protein)-antibody in PBS but would like to ask if she can store antigen-antibody sa...

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Thank you for your enquiry. I am sorry the customer has some concerns regarding the results of their experiment. We would not recommend to store the antigen-antibody samples in distilled water. The sample and the antibody binding are likely to be much...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"