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Goat anti-Rabbit IgG Fc (HRP) secondary antibody (ab97200)

Submit an Abreview Q&A (5)Specific References (1)

Overview

  • Product nameGoat anti-Rabbit IgG Fc (HRP) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - Fc (HRP)
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG.
  • Tested applicationsICC, IHC-P, ELISA, WB more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/200 - 1/2000.
ELISA ELISA: 1/10000 - 1/100000. (Primary ELISA)
WB WB: 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

Goat anti-Rabbit IgG Fc (HRP) secondary antibody images

  • Western blot - Goat anti-Rabbit IgG Fc (HRP) secondary antibody (ab97200)
    Western blot - Goat anti-Rabbit IgG Fc (HRP) secondary antibody (ab97200)
    Ab97200 was used at dilution 1/5000 with the primary antibody ab90045 in WB. See the review on ab90045.

References for Goat anti-Rabbit IgG Fc (HRP) secondary antibody (ab97200)

This product has been referenced in:
  • Knies K  et al. Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges. PLoS One 7:e52648 (2012). WB ; Rabbit . Read more (PubMed: 23285130) »

See 1 Publication for this product

Publishing research using ab97200 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab97200 Submit a Question for ab97200

Displaying 1 - 5 of 5 results for Abreviews and Q&A

10
Votes: Highest First

I will use anti-osteopontin rabbi policlonal ABCAM ab91655 for Western blott purpose. I need a secondary antibody

HRP labelled goat anti-rabbit as suggested by the site.

Should I use ab97200 or ab97069 ? Or maybe another one?


I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:
http://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-a...

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ab7778und Proben vom Schwein

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab7778 bisher noch nicht inSchwein getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot &u...

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Dear All.



Please let us have alternate product information on the items of Life Technologies as below:



Catalogue of competitor-656120

Catalogue of competitor-610120



Sincere re...

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Thank youf or your inquiry.

We have many goat anti rabbit IgG HRP conjugated antibodies:

Please use the links below to review the datasheets of three of them:

ab97051

http://www.abcam.com/index.html?datasheet=97051 ...

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Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I ...

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Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not te...

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antibodies class specific againts

We have been informed by the source of the secondary antibodies ab97195 and ab97200 that : By immunoelectrophoresis and ELISA, ab97195 (anti-Rabbit IgM - HRP) reacts specifically with rabbit IgM. This antibody may cross react with IgM from other species...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"