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Goat anti-Rabbit IgG H&L (10nm Gold) secondary antibody (ab27234)

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1 question for ab27234

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Question 1

Monday 23-April-2012

Dear technical team,

Our customer would like to purchase ab27234 but there's no information about dilution factor, so please let me know if you have information.


Best regards,

ANSWER:

  

Thank you for your enquiry and your interest.

I have discussed your question with an expert onEM technique in the Lab.

The optical density of gold conjugates of this particle size at 520nm wavelength is 3.0. This allows the conjugates to be used in a highly diluted form. The appropriate dilution will vary between applications so the only way to establish the appropriate dilution for a specific application is to create a dilution range and see which dilution gives the best results.

I hope this helps and if I can assist further, please do not hesitate to contact me.

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