Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)
Overview
- Product nameGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)See all IgG secondary antibodies ...
- Target speciesRabbit
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
- Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
- Conjugation notesF/P ratio is 4.85
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab97050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/50 - 1/500. |
| ICC/IF | ICC/IF: 1/50 - 1/500. |
| Flow Cyt | Flow Cyt: 1/50 - 1/200. |
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) images
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Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3% PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibodies:
Mouse anti-Ki67, 1:100
Rabbit anti-laminin alpha 1 (basement marker)
Secondary antibodies:
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AMCA) (ab47052), 1:100
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:100 -
Flow Cytometry - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)The FACS staining was perform on THP-1 cell lines with Rabbit polyclonal to CCR2 (ab21667) at a 1/100 dillution for 30 min at 4C. The secondary antibody was ab97050 used at 1/100 for 20min at 4C. The buffer used was PBS/BSA (0.5%)/Azide (0.05%). No fixation or permeabilization was performed and gating was done on alive cells.
This image is courtesy of an anonymous Abreview
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Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
Nuclei were counterstained stained with DRAQ7TM (ab109202), 1:1000 -
Flow Cytometry - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)The FACS staining was performed on HEK cells expressing human CCR5 with PBS/BSA (0.5%)/Azide (0.05%) as FACS buffer. We used a primary polyclonal rabbit antibody against CCR5 at a 1/100 dillution, incubated for 1 hour at 4C. The secondary antibody was ab97050 used at a concentration of 1/100 for 30min at 4C. No fixation or permeabilization was performed and gaiting was done on alive cells.
This image is courtesy of an anonymous Abreview
References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)
ab97050 has not yet been referenced specifically in any publications.
