Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051)
Overview
- Product nameGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)See all IgG secondary antibodies ...
- Target speciesRabbit
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
- Tested applicationsICC, IHC-P, ELISA, WB more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
- Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
- Clonality Polyclonal
- IsotypeIgG
- General notesPart of the AbExcel range.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab97051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: Use at an assay dependent dilution. |
| IHC-P | IHC-P: 1/200 - 1/5000. |
| ELISA | ELISA: 1/10000 - 1/100000. (Primary). |
| WB | WB: 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000 |
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) images
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Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051)All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051)
This product has been referenced in:
- Hollern DP et al. A mouse model with T58A mutations in Myc reduces the dependence on KRas mutations and has similarities to claudin-low human breast cancer. Oncogene : (2012). Read more (PubMed: 22525269) »
