Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed (ab98418)
- Product nameGoat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesRat
- Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
- Storage instructionsStore at +4°C in the dark.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab98418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: 1/50 - 1/500.|
|ICC/IF||ICC/IF: 1/50 - 1/500.|
|Flow Cyt||Flow Cyt: 1/100 - 1/1000.|
Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed images
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rat anti-perlecan alpha 1, 1:100
Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5®) (ab98418), 1:100
Nuclei were counterstained with SYTO 16
References for Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed (ab98418)
ab98418 has not yet been referenced specifically in any publications.