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Goat anti-Rat IgG H&L (DyLight® 488) secondary antibody (ab96887)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product nameGoat anti-Rat IgG H&L (DyLight® 488) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488)
  • Target speciesRat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Goat anti-Rat IgG H&L (DyLight® 488) secondary antibody images

  • DyLight®-Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488)(ab96887)
    DyLight®-Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488)(ab96887)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat anti-Rat IgG H&L (DyLight® 488) secondary antibody (ab96887)

This product has been referenced in:
  • Lundholm L  et al. Resistance to DNA-damaging treatment in non-small cell lung cancer tumor-initiating cells involves reduced DNA-PK/ATM activation and diminished cell cycle arrest. Cell Death Dis 4:e478 (2013). Flow Cyt ; Human . Read more (PubMed: 23370278) »

See 1 Publication for this product

Publishing research using ab96887 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab96887 Submit a Question for ab96887

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having trouble with staining rat primary and rat tissue



Hello!

The background staining may be due to detection of endogenous immunoglobulins by secondary. I suggest pre blocking the section with unconjugated anti-rat antibodies (or anti mouse if its mouse tissue) to reduce this background. I also sugg...

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What is the concentration of this labeled antibody?

The concentration is 0.5 mg/ml and is provided in a volume of 1 ml.

I hope this is helpful. Please contact us again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"