Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab98420)
- Product nameGoat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesRat
- Tested applicationsFlow Cyt, ICC/IF, IHC-P more details
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
- Storage instructionsStore at +4°C in the dark.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab98420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: 1/50 - 1/200.|
|ICC/IF||ICC/IF: 1/50 - 1/500.|
|IHC-P||IHC-P: 1/50 - 1/500.|
Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed images
Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
ICC/IF image of ab6161 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rat- H&L, pre-adsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab98420)
ab98420 has not yet been referenced specifically in any publications.