Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab98420)

Overview

• Product nameGoat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbedSee all IgG secondary antibodies ...
• Target speciesRat
• Tested applicationsFlow Cyt, ICC/IF, IHC-P more details
• Minimal
  cross-reactivity
  Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep
  To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
  more details

Properties

• FormLiquid
• Storage instructionsStore at +4°C in the dark.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.2% BSA, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Secondary antibodies
  • anti-Rat
  • IgG
  • Fluorophore
  • DyLight® 488

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications

Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed images

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  DyLight® - Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab98420)
  Emission spectra of DyLight® fluorochromes available in our catalog.
  Line colors represent the approximate visible colors of the wavelength maxima.
  
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  Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab98420)
  ICC/IF image of ab6161 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rat- H&L, pre-adsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.