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Goat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (AP), pre-adsorbed (ab102315)

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Overview

  • Product nameGoat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (AP), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesSyrian Hamster
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with syrian hamster IgG and with light chains common to other syrian hamster immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applicationsICC, IHC-P, ELISA, WB more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab102315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/100 - 1/1000.
ELISA ELISA: 1/1000 - 1/10000. ELISA - Primary
WB WB: 1/1000 - 1/10000. WB - Chemiluminescent

References for Goat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (AP), pre-adsorbed (ab102315)

ab102315 has not yet been referenced specifically in any publications.

Publishing research using ab102315 ? Please let us know so that we can cite the reference in this datasheet

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Could you please explain what is a pre-adsorbed antibody? During an ELISA experiment what is the difference between using the ab102305 and the ab102315? Thank you in advance for your attention to this matter.

Thank you for contacting us.

"Pre-adsorbed" refers to purification of the secondary antibody that removes any antibodies in the preparation that might react with immunoglobulins of species other than Syrian Hamster.

The secondary ant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"