Overview

  • Product name
    Golgi Staining Kit - Green Fluorescence - Cytopainter
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mammal
  • Product overview

    Golgi Staining Kit - Green Fluorescence | Cytopainter (ab139483) contains an Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. Micromolar concentrations of the green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat, and human bone osteosarcoma epithelial cell line, U2OS. One important application of ab139483 is in fluorescence co-localization imaging with red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.


    ab139483 is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.

  • Notes

    This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the FITC region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab139483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Golgi Green Detection dye co-localizes with N-acetylgalactosaminyltransferase-2, fused to red fluorescent protein (RFP). (A) Golgi Green Detection dye, (B) HeLa cells expressing RFP-N-acetylgalactosaminyltransferase-2, (C) Hoechst 33342 staining, (D) composite image.
  • Fluorescence excitation and emission spectra for Golgi Green Detection dye dye (panel A) and absorbance and fluorescent emission spectra for Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Solution.

Protocols

References

This product has been referenced in:
  • Aasen SN  et al. A Novel Nanoprobe for Multimodal Imaging Is Effectively Incorporated into Human Melanoma Metastatic Cell Lines. Int J Mol Sci 16:21658-80 (2015). Read more (PubMed: 26370983) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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