Anti-Granulocytes antibody [HIS48] (ab33760)
Key features and details
- Mouse monoclonal [HIS48] to Granulocytes
- Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Rat
- Isotype: IgM
Overview
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Product name
Anti-Granulocytes antibody [HIS48]
See all Granulocytes primary antibodies -
Description
Mouse monoclonal [HIS48] to Granulocytes -
Host species
Mouse -
Specificity
ab3760 recognises granulocytes and erythroid cells. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat Granulocytes. PVG rat spleen cell suspension.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 8% Fetal calf serum, 3.16% Tris HCl -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
HIS48 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab33760 has been referenced in 6 publications.
- Bao L et al. Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution. Bioact Mater 9:183-197 (2022). PubMed: 34820565
- Cerullo D et al. Add-On Cyclic Angiotensin-(1-7) with Cyclophosphamide Arrests Progressive Kidney Disease in Rats with ANCA Associated Glomerulonephritis. Cells 11:N/A (2022). PubMed: 35954280
- Cerullo D et al. Characterization of a Rat Model of Myeloperoxidase-Anti-Neutrophil Cytoplasmic Antibody-Associated Crescentic Glomerulonephritis. Nephron 145:428-444 (2021). PubMed: 33910203
- Büttner P et al. Arginine metabolism and nitric oxide turnover in the ZSF1 animal model for heart failure with preserved ejection fraction. Sci Rep 11:20684 (2021). PubMed: 34667218
- Markworth JF et al. Resolvin D1 supports skeletal myofiber regeneration via actions on myeloid and muscle stem cells. JCI Insight 5:N/A (2020). PubMed: 32750044
- Ma S et al. Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging. Cell 180:984-1001.e22 (2020). PubMed: 32109414