Granulocytes antibody [0.N.315] - BSA and Azide free (ab14499)

Product Name 

Granulocytes antibody [0.N.315] - BSA and Azide free

Product type 

Primary antibodies

Description 

Mouse monoclonal [0.N.315] to Granulocytes - BSA and Azide free

Immunogen 

Nuclei from pokeweed mitogen-stimulated human peripheral blood lymphocytes.

Reacts with 

Hu

Does not react with 
(species key)

Rat

Specificity 

Recognizes an unidentified antigen in the cytoplasm of mature granulocytes.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml. No special pretreatment required.
IHC-Fr: Use at a concentration of 2 - 4 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Immunology >> Innate Immunity >> Myeloid Cells
Immunology >> Cell Type Markers >> Non-CD >> APC

Relevance 

Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells.
Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

0.N.315

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: 10mM PBS, pH 7.4

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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